Plan
Decide what goes where before you start.
Prepare
At the bench: solutions, mixes, spins and cells.
Solutions and concentrations
Reactions and equipment
Analyse
Readings in, answers out.
DNA, RNA and protein
What's free and what's Pro
All the calculators, the plate layout designer, fitting a single curve and the IC50 calculator are free, and they'll stay free. Pro is meant for people who analyse assays every week: it adds comparing several curves, importing readings through the plate layout, and downloading figures from curve fitting and the IC50 calculator. Those features carry a Pro label and can be used free while Pro is in early access. I'll announce pricing well before early access ends.
Accuracy
Before each release, every calculator is run through test cases whose answers were worked out separately, and the curve fitting and IC50s are compared with SciPy. Any result can show its working, unit conversions included, if you want to check the arithmetic yourself. Answers are shown to 4 significant figures, but every step is calculated at full precision.
The tests check the arithmetic, not your protocol. Constants such as A260 factors or a rotor's radius are worth confirming against your own SOPs. There's more detail on how the calculators are checked, and if a result looks wrong, email [email protected] with the numbers you entered.
Who makes it
I'm Steven Lamont. I've spent 20 years developing and testing lateral flow diagnostics and work as a Technical Director in that industry. WellSpotted is my own project, separate from that job. About WellSpotted.
Privacy
The calculations run in your browser, so what you type isn't sent anywhere. The only things that reach me are what you choose to send through the feedback form and anonymous daily counts of which tools are used. There are no accounts and no tracking cookies. Privacy.